Journal: PLoS ONE

Article Title: An Integrative Analysis to Identify Driver Genes in Esophageal Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0139808

Figure Lengend Snippet: GISTIC-defined peaks associated with altered expression in ESCC.

Article Snippet: ERBB2 and GRB7 proteins were detected using an anti-ERBB2 rabbit monoclonal antibody (Epitomics, Inc) at a 1:5000 dilution and an anti-GRB7 rabbit monoclonal antibody (Gene Tex International Corporation) at a 1:1000 dilution.

Techniques: Expressing, Amplification

Journal: PLoS ONE

Article Title: An Integrative Analysis to Identify Driver Genes in Esophageal Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0139808

Figure Lengend Snippet: Results of analysis on RNA interference screening data of Project Achilles.

Article Snippet: ERBB2 and GRB7 proteins were detected using an anti-ERBB2 rabbit monoclonal antibody (Epitomics, Inc) at a 1:5000 dilution and an anti-GRB7 rabbit monoclonal antibody (Gene Tex International Corporation) at a 1:1000 dilution.

Techniques: Virus

Journal: PLoS ONE

Article Title: An Integrative Analysis to Identify Driver Genes in Esophageal Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0139808

Figure Lengend Snippet: (a) Reductions in mRNA and protein levels of GRB7 at 48 hours after siRNA transfection in KYSE410 and TE4 cells. The results are the mean ± SD from 3 replicates of a single experiment. (b) GRB7 inactivation reduced proliferation of KYSE410 and TE4 cells. Cell growth was measured on days 2, 3, and 4 by MTT assay. Absorbance at day 0 was assigned a value of 1. The results are the mean ± SD from 6 replicates of a single experiment. (c) Migration and invasion assays using GRB7 -knockdown cells. Each bar represents the average of 3 measurements. (d) Inhibitory effects of siRNA targeting GRB7 in combination with trastuzumab. Cells were transfected with siRNA targeting GRB7 or negative control siRNA and treated with or without trastuzumab (0.1 and 1.0 μg/mL). Cells were then seeded in 96-well plates, and cell growth was monitored every 24 hours using MTT assays. Absorbance at day 0 was assigned a value of 1. The results are the mean ± SD from 6 replicates of a single experiment.

Article Snippet: ERBB2 and GRB7 proteins were detected using an anti-ERBB2 rabbit monoclonal antibody (Epitomics, Inc) at a 1:5000 dilution and an anti-GRB7 rabbit monoclonal antibody (Gene Tex International Corporation) at a 1:1000 dilution.

Techniques: Transfection, MTT Assay, Migration, Knockdown, Negative Control

Journal: PLoS ONE

Article Title: An Integrative Analysis to Identify Driver Genes in Esophageal Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0139808

Figure Lengend Snippet: (a) Analysis of GRB7 mRNA expression in tumor tissues and the corresponding normal mucosa by real-time RT-PCR. (b) Kaplan-Meier survival curves for ESCC patients according to GRB7 mRNA expression.

Article Snippet: ERBB2 and GRB7 proteins were detected using an anti-ERBB2 rabbit monoclonal antibody (Epitomics, Inc) at a 1:5000 dilution and an anti-GRB7 rabbit monoclonal antibody (Gene Tex International Corporation) at a 1:1000 dilution.

Techniques: Expressing, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: An Integrative Analysis to Identify Driver Genes in Esophageal Squamous Cell Carcinoma

doi: 10.1371/journal.pone.0139808

Figure Lengend Snippet: Results of univariate and multivariate analysis of clinicopathlogical factors for 5-year overall survival in the validation set.

Article Snippet: ERBB2 and GRB7 proteins were detected using an anti-ERBB2 rabbit monoclonal antibody (Epitomics, Inc) at a 1:5000 dilution and an anti-GRB7 rabbit monoclonal antibody (Gene Tex International Corporation) at a 1:1000 dilution.

Techniques: Biomarker Discovery, Expressing

Journal: Oncotarget

Article Title: The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

doi: 10.18632/oncotarget.27593

Figure Lengend Snippet: Figure 1: GRB7 expression in normal breast and ovarian tissues. Representative images of normal tissues stained with the GRB7 antibody (brown) and Hematoxylin (blue). (A) Normal breast tissue showing no GRB7 expression in the mammary ducts. (B) Normal breast tissue showing high GRB7 expression (3+) in the myoepithelial cells of mammary ducts. (C) Normal ovarian surface epithelium showing strong cytoplasmic GRB7 (3+) expression. (D) Normal ovarian tissue showing no GRB7 expression in the stroma. (E) Normal fallopian tubes with weak GRB7 (1+) expression. All images were taken with a 40× objective (scale bar 50 µm).

Article Snippet: anti-human GRB7, rabbit monoclonal antibody (Abcam, Cat# 183737 [EPR14099]); anti α-tubulin, mouse Oncotarget2287www.oncotarget.com monoclonal antibody (Cell signaling, Danvers, MA, USA, Cat# 3873s); anti HER-2/ErbB2, rabbit polyclonal antibody (Cell signaling, Cat# 2242).

Techniques: Expressing, Staining

Journal: Oncotarget

Article Title: The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

doi: 10.18632/oncotarget.27593

Figure Lengend Snippet: Figure 2: GRB7 expression in benign fibroadenoma and fibrocystic changes. Examples of images of benign breast cancer tumors stained with the GRB7 antibody (brown) and Hematoxylin (blue). (A) Benign fibroadenoma tissue showing high GRB7 expression (3+) in myoepithelial cells. (B) Benign fibrocystic changes showing no GRB7 expression (0). Images were taken with a 40 × objective, scale bar = 50 µm).

Article Snippet: anti-human GRB7, rabbit monoclonal antibody (Abcam, Cat# 183737 [EPR14099]); anti α-tubulin, mouse Oncotarget2287www.oncotarget.com monoclonal antibody (Cell signaling, Danvers, MA, USA, Cat# 3873s); anti HER-2/ErbB2, rabbit polyclonal antibody (Cell signaling, Cat# 2242).

Techniques: Expressing, Staining

Journal: Oncotarget

Article Title: The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

doi: 10.18632/oncotarget.27593

Figure Lengend Snippet: Figure 3: GRB7 expression and localization in invasive ductal carcinoma (IDC) of the breast. Representative images of IDC of the breast tissues stained with the GRB7 antibody (brown) and Hematoxylin (blue). (A) IDC showing no GRB7 expression (0). (B) IDC showing weak cytoplasmic GRB7 expression (1+). (C) IDC showing medium cytoplasmic GRB7 expression (2+). (D) IDC showing strong cytoplasmic GRB7 expression (3+). (E) IDC showing strong cytoplasmic GRB7 expression with membranous accentuation (3+). Images were taken with a 40 × objective, scale bar = 50 µm.

Article Snippet: anti-human GRB7, rabbit monoclonal antibody (Abcam, Cat# 183737 [EPR14099]); anti α-tubulin, mouse Oncotarget2287www.oncotarget.com monoclonal antibody (Cell signaling, Danvers, MA, USA, Cat# 3873s); anti HER-2/ErbB2, rabbit polyclonal antibody (Cell signaling, Cat# 2242).

Techniques: Expressing, Staining

Journal: Oncotarget

Article Title: The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

doi: 10.18632/oncotarget.27593

Figure Lengend Snippet: Figure 4: GRB7 expression in ovarian carcinoma cells. Representative images of high grade ovarian cancer tissues stained with the GRB7 antibody (brown) and Hematoxylin (blue). (A) No GRB7 expression (0). (B) Weak cytoplasmic GRB7 expression (1+). (C) Medium cytoplasmic GRB7 expression (2+). (D) Strong cytoplasmic GRB7 expression (3+). (E) Strong cytoplasmic GRB7 expression with membranous accentuation (3+). Images were taken with a 40 × objective, scale bar = 50 µm.

Article Snippet: anti-human GRB7, rabbit monoclonal antibody (Abcam, Cat# 183737 [EPR14099]); anti α-tubulin, mouse Oncotarget2287www.oncotarget.com monoclonal antibody (Cell signaling, Danvers, MA, USA, Cat# 3873s); anti HER-2/ErbB2, rabbit polyclonal antibody (Cell signaling, Cat# 2242).

Techniques: Expressing, Staining

Journal: Oncotarget

Article Title: The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

doi: 10.18632/oncotarget.27593

Figure Lengend Snippet: Figure 5: Kaplan-Meier survival analysis for GRB7 membrane-associated staining from 173 patients with ovarian cancer. (A) Recurrence Free Survival (RFS) and (B) Overall Survival (OS) curves for each patient showing GRB7 membrane-associated expression shown as yes (black curve)/no (gray curve) for each patient). (C) Landmark analysis for RFS (shown in (A) using 810 days (27 months) as the cut off mark (dotted lined). (D) RFS and (E) OS curves for patients with at least two out of three tissue cores with GRB7 membrane-associated expression (black curve) vs. one or no GRB7 membrane-associated expression (gray curve). All survival curves were generated after dichotomization of GRB7 membrane-associated expression (black curves, “GRB7 yes”) or no GRB7 membrane-associated expression (gray curve, “GRB7 no”), obtained by IHC. p-values were calculated by log-rank tests.

Article Snippet: anti-human GRB7, rabbit monoclonal antibody (Abcam, Cat# 183737 [EPR14099]); anti α-tubulin, mouse Oncotarget2287www.oncotarget.com monoclonal antibody (Cell signaling, Danvers, MA, USA, Cat# 3873s); anti HER-2/ErbB2, rabbit polyclonal antibody (Cell signaling, Cat# 2242).

Techniques: Membrane, Staining, Expressing, Generated, Paraffin-embedded Immunohistochemistry

Journal: Oncotarget

Article Title: The prognostic significance of GRB7 protein expression and localization in human breast and ovarian cancers.

doi: 10.18632/oncotarget.27593

Figure Lengend Snippet: Figure 6: GRB7 expression levels and localization of breast cancer cell lines (MDA-MB-231, HCC1954, and SKBR3). (A) Western blot showing GRB7 expression levels (middle) panel using total protein lysates (10 µg/lane). Alpha-tubulin (aTUB, bottom panel) was used as internal reference, HER-2 was used to show co-amplification of HER-2 and GRB7 as expected (top panel). MD = MDA-MB-231, HC = HCC1954, SK = SKBR3. (B) Representative images of breast cancer (HCC1954, SKBR3) cell lines stained (IHC) with the GRB7 antibody (brown, developed using HRP-DAB) and nuclei (Hematoxylin, blue). Images show cells with strong cytoplasmic GRB7 expression with membranous accentuation. (C) Representative images of breast cancer (HCC1954, SKBR3) cell lines stained (IF) with the GRB7 antibody (green), WGA cell membrane marker (magenta), nuclei (blue), and a merged image from all three channels, showing GRB7 and WGA co-localization in white (most right). A DIC image is shown on the most left. Images were taken with a 20 × objective, scale bar = 100 µm.

Article Snippet: anti-human GRB7, rabbit monoclonal antibody (Abcam, Cat# 183737 [EPR14099]); anti α-tubulin, mouse Oncotarget2287www.oncotarget.com monoclonal antibody (Cell signaling, Danvers, MA, USA, Cat# 3873s); anti HER-2/ErbB2, rabbit polyclonal antibody (Cell signaling, Cat# 2242).

Techniques: Expressing, Western Blot, Amplification, Staining, Membrane, Marker

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Expression of Grb7 family members in PN21 mouse pancreas visualised by immunohistochemistry on paraffin sections. Antibodies specific for ( A ) Grb7; B Grb10; and C Grb14; were used to detect distinct proteins of the Grb7 family. Brown staining is indicative of protein expression. Arrows indicate endocrine area (pancreatic islets); magnification 100x

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Weights and blood glucose levels in PN1 progeny from Grb10 KO x Grb14 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb10 KO and Grb14 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb10 KO (10KO), Grb14 KO (14KO) and Grb10 : Grb14 double knockout (DKO). For each of the four offspring genotype groups, data are shown for ( A ) Body weight; and B Blood glucose concentration ([Glu]). In addition, actual weights of ( C ) Brain; D Liver; E Lungs; F Heart; and G Kidneys; are shown above the relative weights of the same organs, expressed as a percentage of body mass ( H – L ). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for total body and all organs, WT N = 25, Grb10 KO N = 13, Grb14 KO N = 7, Grb10 : Grb14 DKO N = 7; glucose levels, WT N = 18, Grb10 KO N = 9, Grb14 KO N = 6, Grb10 : Grb14 DKO N = 4. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques: Double Knockout, Concentration Assay

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Genetic crosses used in the study, showing parent and offspring genotypes with their expected ratios. Crosses between (A) Grb10 +/p : Grb14 +/- double heterozygous females and Grb10 +/p : Grb14 +/- double heterozygous males, producing offspring of twelve genotypes, and (B) Grb7 +/- : Grb10 +/p double heterozygous females and Grb7 +/- heterozygous males, producing offspring of six genotypes, each in the indicated, expected Mendelian ratios. For ANOVA statistical analysis these six or twelve genotypes were used to form four groups, as indicated, since no difference was anticipated between animals differing only in their Grb14 +/- and Grb14 +/+ or Grb7 +/- and Grb7 +/+ allelic status. Similarly, due to the imprinted expression of Grb10 , offspring inheriting a mutant copy of the paternal Grb10 allele ( Grb10 +/p ), having no growth phenotype, were not expected to differ from Grb10 wild type ( Grb10 +/+ ) offspring, while double knockout (DKO) offspring inheriting mutations of both parental alleles ( Grb10 m/p ) were expected to be indistinguishable from those inheriting a mutant copy of the normally active maternal Grb10 allele ( Grb10 m/+ )

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques: Expressing, Mutagenesis, Double Knockout

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Summary of PN1 body and organ weight data for progeny of crosses between (A) the Grb10 KO and Grb14 KO strains, and (B) the Grb7 KO and Grb10 KO strains. Mean weights are shown for each genotype together with changes relative to wild type (%WT) for each mutant genotype

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques: Mutagenesis

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Weights and blood glucose levels in PN1 progeny from Grb7 KO x Grb10 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockout (DKO). For each of the four offspring genotype groups, data are shown for, A Body weight; and B Blood glucose concentration ([glu]). In addition, actual weights of ( C ) Brain; D Liver; E Lungs; F Heart and G Kidneys are shown above the relative weights of the same organs, expressed as a percentage of body mass ( H – L ). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for body and brain, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; kidneys and heart, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 48, Grb7 : Grb10 DKO N = 15, liver, WT N = 42, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 14; lungs, WT N = 41, Grb7 KO N = 14, Grb10 KO N = 49, Grb7 : Grb10 DKO N = 15; glucose levels, WT N = 24, Grb7 KO N = 7, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 10. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques: Double Knockout, Concentration Assay

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Weight analysis of e17.5 conceptuses from crosses between Grb7 KO and Grb10 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb7 KO (7KO), Grb10 KO (10KO) and Grb7 : Grb10 double knockouts (DKO). Weights are shown for the four offspring genotype groups for ( A ) Embryo; and B Placenta. C These values have been used to calculate the embryo to placenta weight ratio as a measure of placental efficiency. Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for embryo WT N = 24, Grb7 KO N = 5, Grb10 KO N = 27, Grb7 : Grb10 DKO N = 6, and for placenta and embryo:placenta ratio WT N = 23, Grb7 KO N = 5, Grb10 KO N = 27, Grb7:Grb10 DKO N = 6. Asterisks indicate p- values, * p < 0.05, ** p < 0.01, **** p < 0.0001

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques:

Journal: BMC Biology

Article Title: Grb7 , Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

doi: 10.1186/s12915-024-02018-5

Figure Lengend Snippet: Grb7, Grb10 and Grb14 distribution in the developing mouse embryo

Article Snippet: For immunohistochemistry, protocols were essentially as previously described [ ], using primary antibodies specific for either Grb7 (Santa Cruz Biotechnology, CA, USA; GRB7 (N-20) N-terminal Grb7, rabbit polyclonal sc-607; RRID:AB_2113275), at 1:200 dilution, Grb10 (Santa Cruz Biotechnology, CA, USA; GRB10 (A-18) C-terminal α-Grb10, rabbit polyclonal sc-1027) at 1:200 dilution or Grb14 (Santa Cruz Biotechnology, CA, USA; GRB14 (N-19) N-terminal Grb14, goat polyclonal sc-6103; RRID:AB_2112989) at 1:100 dilution.

Techniques: